Biology Calculators (Page 2)
Genetics, ecology and physiology metrics — biology calculations.
Cell Density Calculator
Enter the average cell count per large square of a hemocytometer, the dilution factor, and the sample volume; the tool computes cell density (cells/mL) and total cell count, for cell culture and passaging counts.
MOI Calculator
Enter the number of virus particles and the number of host cells to compute the multiplicity of infection (MOI), the average number of virions per cell.
Michaelis–Menten Calculator
Enter maximum velocity Vmax, Michaelis constant Km, and substrate concentration [S] to compute the enzyme-catalyzed reaction rate v and its fraction of Vmax, for enzyme-kinetics analysis.
RNA Copy Number Calculator
Enter RNA mass, nucleotide length, and average molar mass per nucleotide to compute RNA molecule copy number, for qRT-PCR standards and IVT quantification.
OD600 Cell Density Calculator
Enter the optical density at 600 nm (OD600), conversion factor, and culture volume to estimate bacterial cell density (cells/mL) and total cell count, for monitoring bacterial cultures.
GC Content Calculator
Enter the counts of A, T, G, C bases in a DNA sequence to compute the GC percentage and an estimated melting temperature (Tm), for primer design and sequence analysis.
Primer Tm Calculator
Enter the counts of A, T, G, C in a primer to compute the Wallace-rule and salt-corrected (GC%) melting temperature Tm, for PCR primer design.
Enzyme Activity Calculator
Enter the absorbance slope ΔA/min, molar extinction coefficient ε, path length, reaction and sample volumes, and protein concentration to compute enzyme activity (U/mL) and specific activity (U/mg).
qPCR Efficiency Calculator
Enter the slope of the qPCR standard curve to compute amplification efficiency E = 10^(−1/slope) − 1, evaluating reaction performance and whether the standard curve is ideal.
Water Potential Calculator
Enter solute potential Ψs and pressure potential Ψp to compute total water potential Ψ and determine osmotic water-flow direction, for plant physiology and osmosis study.
Feed Conversion Ratio Calculator
Enter feed intake and weight gain to compute the feed conversion ratio (FCR) and feed efficiency (FE), evaluating how efficiently livestock and aquaculture convert feed into body mass.
Animal Mortality Rate Calculator
Enter deaths and starting total to compute mortality rate, survival rate, and survivor count — for farmed stocks, disease surveillance, and ecological surveys.