Dilution Calculator
Enter the stock concentration, target concentration and target volume; using C₁V₁ = C₂V₂ the tool instantly computes the required stock (concentrate) volume, the solvent (water) volume to add and the dilution factor.
Input Data
Results
At a glance:Dilution is the operation of adding solvent (usually water) to a concentrated stock solution to lower its concentration and increase its volume; it is the most basic step in preparing standards, buffers and reagents in the lab. The core of dilution is 'conservation of solute': you only add solvent, never solute, so the total moles (or mass) of solute are unchanged before and after dilution — only spread over a larger volume, so the concentration falls. Written as a formula this is the famous C₁·V₁ = C₂·V₂, where C₁, V₁ are the concentration and volume before dilution (stock) and C₂, V₂ after dilution (target solution). On both sides C×V represents 'amount of solute' because concentration times volume cancels the volume unit and yields amount. The most common practical question: you have stock at concentration C₁ and want to prepare a solution of concentration C₂ and volume V₂ — how much stock do you take? Rearranging gives V₁ = C₂·V₂ / C₁, which is the 'required stock volume' this calculator returns. After taking the stock, add solvent up to V₂; the solvent (water) volume to add = V₂ − V₁. The 'dilution factor' = C₁ / C₂ = V₂ / V₁ represents how many times the solution is diluted; for example diluting 2 M to 0.5 M is a 4-fold dilution (written 1:4, i.e. 1 part stock to 4 parts solution). Using this tool's default: stock C₁ = 2 M, want V₂ = 100 mL of C₂ = 0.5 M, take V₁ = 0.5 × 100 / 2 = 25 mL, then add 100 − 25 = 75 mL water, dilution factor = 2 / 0.5 = 4. Notes on using the dilution formula: first, the units of C₁ and C₂ must be consistent (both mol/L, or both %, mg/mL), and V₁ and V₂ must share a volume unit; the formula does not restrict the unit type, only that both sides use the same kind and unit. Second, the target concentration C₂ cannot exceed the stock C₁ — adding solvent alone cannot make a solution more concentrated; if C₂ > C₁ you need a more concentrated stock or evaporation, and this calculator will flag that dilution is impossible. Third, the correct practice is 'take stock first, then add solvent to the mark', not measure water first then add stock, because the dissolved solute also occupies volume; especially when diluting concentrated acids or bases, always add acid to water to avoid violent exothermic splashing. Fourth, the formula assumes additive volumes and a non-reacting solute; for highly concentrated solutions with noticeable volume contraction (e.g. concentrated sulphuric acid) it is only approximate. Dilution is the basis of serial dilution and molarity conversion: a serial dilution repeatedly applies C₁V₁=C₂V₂ to make a concentration gradient, used in microbiology counting and standard-curve preparation. In short, the dilution calculator quantifies 'how much stock, how much water' into a single step — a daily tool in chemistry, biology, medical laboratory and pharmacy preparation.
Formula
Dilution conservation: C₁ · V₁ = C₂ · V₂ (amount of solute unchanged).
Required stock volume: V₁ = C₂ · V₂ ÷ C₁.
Solvent (water) volume to add = V₂ − V₁.
Dilution factor = C₁ ÷ C₂ = V₂ ÷ V₁.
$$C_1 V_1 = C_2 V_2$$$$V_1 = \dfrac{C_2 V_2}{C_1}$$How to Use
- Enter the concentration C₁ of your stock (concentrate).
- Enter the target concentration C₂ (must be ≤ C₁) and the target total volume V₂.
- The right panel instantly shows the required stock volume V₁, the solvent (water) volume to add and the dilution factor.
Common dilution factors and preparation ratios (target volume 100 mL)
| Stock C₁ | Target C₂ | Dilution Factor | Stock V₁ | Water Added |
|---|---|---|---|---|
| 2 M | 1 M | 2× | 50 mL | 50 mL |
| 2 M | 0.5 M | 4× | 25 mL | 75 mL |
| 2 M | 0.2 M | 10× | 10 mL | 90 mL |
| 1 M | 0.1 M | 10× | 10 mL | 90 mL |
| 10 M | 0.5 M | 20× | 5 mL | 95 mL |
Dilution factor = C₁/C₂; stock volume = C₂·V₂/C₁; water added = V₂ − V₁ (target volume V₂ fixed at 100 mL).
Case Studies
Preparing a 0.5 M NaCl dilution
You have a 2 M sodium chloride stock and want 100 mL of 0.5 M solution.
Required stock V₁ = C₂·V₂/C₁ = 0.5 × 100 / 2 = 25 mL, then add 100 − 25 = 75 mL distilled water to make up to 100 mL.
Dilution factor = 2 / 0.5 = 4-fold (1:4); check: post-dilution solute = 0.5 × 100 = 50 equals stock taken 2 × 25 = 50, conservation holds.
10× concentrated buffer
The lab keeps a 10× concentrated buffer (relative C₁ = 10) and needs 200 mL of 1× working solution.
Required stock V₁ = 1 × 200 / 10 = 20 mL, add water 200 − 20 = 180 mL; 10-fold dilution.
This is the 'take 1 part concentrate plus 9 parts water' 1:10 preparation, the daily standard for making TAE, PBS and similar buffers in molecular biology.
FAQ
Why does C₁V₁ = C₂V₂ hold?
Because dilution adds only solvent, not solute, the total amount of solute (concentration × volume) is conserved before and after. C×V has units of concentration times volume, exactly equal to moles or mass of solute, so pre-dilution C₁V₁ must equal post-dilution C₂V₂.
Must the concentration unit be mol/L?
Not necessarily. C₁V₁=C₂V₂ imposes no unit-type restriction as long as C₁ and C₂ use the same concentration unit (mol/L, %, mg/mL, ppm all work) and V₁ and V₂ use the same volume unit. The factor relationship is independent of units.
Why is the water added V₂ − V₁ rather than V₂?
The target volume V₂ is the total volume of the diluted solution and already includes the stock volume V₁ taken. So the solvent to add only brings the volume from V₁ up to V₂, i.e. V₂ − V₁. The correct practice is to add stock first, then solvent to the V₂ mark.
Can the target concentration be higher than the stock?
No. Adding solvent alone cannot make a solution more concentrated; dilution only lowers concentration. To get a higher concentration you must use a more concentrated stock or concentrate by evaporation or adding solute. When C₂ > C₁ this calculator flags that dilution is impossible (stock volume 0).
What to watch when diluting concentrated acids?
When diluting concentrated sulphuric or hydrochloric acid, always add acid to water — pour the acid slowly into a large amount of water and stir to dissipate heat. Never add water to concentrated acid, otherwise violent local heating can boil and splash, causing burns. Also, highly concentrated solutions are poorly additive in volume, so C₁V₁=C₂V₂ is only approximate.
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References
Content review: Calculatorism Science Team. Results are for reference only; please refer to the relevant authorities for the official figures.